Hematoxylin and Eosin Tissue Stain in Mohs Micrographic Surgery: A Review

BACKGROUND
Several factors may affect Mohs micrographic surgery (MMS) tissue section quality. Although other factors may affect section integrity and ease of processing, tissue stains are the cornerstone of histologic diagnosis. When performed incorrectly, visualization and discrimination of microscopic details may be suboptimal and even impossible. Patient harm may result if an accurate diagnosis cannot be made.


OBJECTIVE
To review hematoxylin and eosin (H&E) technique, the most commonly used staining technique in MMS tissue preparation.


MATERIALS AND METHODS
Review of literature. Tissue sections were H&E stained using a linear stainer where one ...

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