Background: Recently, evidence has been obtained to provoke inflammation through up-regulation of macrophage migration inhibitory factor (MIF) expression by steroids. However, little is known regarding the effect of steroids on MIF expression in human keratinocytes and the counter-effect of EGCG.
Objectives: We determined whether or not steroids cause the up-regulation of MIF in human keratinocytes, and if so, whether or not epigallocatechin-3-gallate (EGCG) suppresses MIF up-regulation in keratinocytes by steroids. We then assessed the effects of EGCG on MIF-induced Th-related chemokine and cytokine expression in keratinocytes.
Methods: HaCaT keratinocytes were first treated ...
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