Noninvasive estimation of chronological and photoinduced skin damage using Raman spectroscopy and principal component analysis

Background

Skin aging can be attributed to endogenous and exogenous factors which modify the hydration and protein structure of the skin which can be measured using Raman spectroscopy.



Method

This study included 21 healthy adult volunteers, aged 32–81 years, Raman spectra were obtained from sun-protected and sun-exposed skin, also three millimeter punch biopsies of sun-exposed skin were collected and analyzed. The Raman spectra were analyzed using principal component analysis and the results were correlated with clinical and histological findings.



Results

The principal component analysis of the Raman spectra shows that the first principal component (PC1) obtained ...

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