Tuesday, October 4, 2011

Altered microRNA expression in mycosis fungoides

Abstract

Background: MicroRNAs are small non-coding RNA molecules involved in the regulation of various physiological and pathological processes. The altered expression of different microRNAs was observed in both, solid tumors and hematological malignancies.


Objectives: The aim of our study was to investigate expression of several microRNAs in early and advanced mycosis fungoides (MF).


Methods: The biopsies were obtained from 43 patients with MF (18 early MF and 25 advanced MF) and 23 healthy volunteers. After microRNA isolation, reverse transcriptase reactions was performed, followed by cDNA amplification. Following microRNA were analyzed: miR-15a, miR-16, miR-155, ...

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