Monday, March 14, 2011

Induction of fat cell necrosis in human fat tissue after treatment with phosphatidylcholine and deoxycholate

Abstract

Background  Injections with phosphatidylcholine- and deoxycholate-containing substances are used to treat localized fat accumulation and lipomas. It is believed that the injected substances induce fat cell destruction with subsequent acute panniculitis followed by a repair process of the treated fat tissue.


Objectives  We investigated whether necrosis or apoptosis of fat cells was induced by the injected substances.


Methods  Samples of fat tissue of lipoma were collected at various times after injection and evaluated by light and electron microscopy, by immunostaining for active caspase-3 and antideoxyribonuclease I, in situ end-labelling (TUNEL staining), and ...

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